5-FAM, SE is the amine-reactive succinimidyl ester of single isomer 5-FAM acid.It is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate such as FITC.
||5-FAM NHS Ester
||5-Carboxyfluorescein, succinimidyl ester
||For Research Use Only