NIR-ASM is a NAD(P)H quinone oxidoreductase 1 (NQO1)-activatable NIR fluorescent probe. It is a conjugate between dicyanoisophorone (ASM) fluorophore and the NQO1 substrate quinone propionic acid (QPA). NIR-ASM remained non-fluorescent until activation by NQO1, whose expression is largely limited to malignant tissues. It has a large Stokes shift (186 nm) and a prominent near-infrared emission (646 nm) in response to NQO1. NIR-ASM is capable of monitoring NQO1 activity in vitro and in vivo with high specificity and selectivity. NIR-ASM has been demonstrated to differentiate cancer cells from normal cells based on NQO1 activity using fluorescence microscopy and flow cytometry. Excitation maximum = 465 nm; emission maximum = 646 nm. Reference: Punganuru, S. R., Madala, H. R., Arutla, V., Zhang, R., & Srivenugopal, K. S. (2019). Characterization of a highly specific NQO1-activated near-infrared fluorescent probe and its application for in vivo tumor imaging. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-44111-8
| Product Name | NIR-ASM |
| Synonyms | |
| CAS Number | 2646628-35-3 |
| Related CAS | |
| Molecular Formula | C33H35N3O3 |
| Molecular Weight | 521.661 |
| Purity (HPLC) | >= 95% |
| Shipping Conditions | Shipped on gel packs |
| Storage Conditions | Store at -20°C and protected from light |
| Regulatory Statement | For Research Use Only |