CP-Alexa Fluor 488
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SNAP-tag is a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. At 20 kDa, SNAP-tag is smaller than HaloTag (33 kDa), potentially reducing interference with the tagged protein’s function. SNAP-tag reacts with covalently with benzylguanine (BG)or chloropyrimidine (CP) derivatives, forming a stable thioether bonds that ensures durability for long-term experiments or harsh conditions. SNAP-tag can be used alongside other tags like CLIP-tag for multicolor labeling. Chloropyrimidine (CP) derivatives generally exhibit better cell permeability compared to their benzylguanine (BG) derivatives. CP-Alexa Fluor 488 is a SNAP-tag substrate containg Alexa Fluor 488 dye. It forms a covalent bond with the SNAP-tag protein, ensuring stable labeling for long-term tracking. Alexa Fluor 488 (494Ex/517Em) is a popular green fluorescent dye known for its brightness, stability, and versatility in various biological and biochemical applications.
| Product Name | CP-Alexa Fluor 488 |
| Synonyms | SNAP2-Alexa Fluor 488 |
| CAS Number | |
| Related CAS | |
| Molecular Formula | C33H23ClN6O11S2 |
| Molecular Weight | 779.15 |
| Purity (HPLC) | >= 95% |
| Solubility | DMSO, DMF |
| Shipping Conditions | Shipped on gel packs |
| Storage Conditions | Store at -20°C and protected from light |
| Shelf Life | 12 months after the date of delivery |
| Regulatory Statement | For Research Use Only |
References
Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00264
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