BC-DBCO

CLIP-tag is a modified version of the SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Unlike the SNAP-tag, which reacts with benzylguanine (BG)or chloropyrimidine (CP) derivatives, the CLIP-tag is engineered to specifically interact with benzylcytosine (BC)derivatives. This modification allows for distinct labeling applications, enabling researchers to use both SNAP-tag and CLIP-tag in the same experiment, providing flexibility in protein labeling and tracking. BC-DBCO is a CLIP-tag substrate containg DBCO moiety for click chemustry apploications. DBCO, or dibenzocyclooctyne, is a key reagent in click chemistry, specifically used in the strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. In SPAAC, DBCO reacts with an azide group to form a stable triazole ring, linking two molecules covalently. Unlike the classic copper-catalyzed azide-alkyne cycloaddition (CuAAC), which uses copper(I) to accelerate the reaction between a terminal alkyne and an azide, SPAAC is copper-free. The strain in DBCO’s cyclooctyne ring drives the reaction forward by releasing energy when it transitions to the triazole product, eliminating the need for a catalyst. This is a big advantage in living systems, where copper can be toxic or interfere with biological processes.

References

Documents 

Datasheet          MSDS

Citations

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