Halo-DBF

Halo-DBF is a HaloTag-reactive dibromofluorescein photosensitizer designed for spatially controlled biomolecule labeling in living cells. Upon covalent binding to a HaloTag fusion protein, Halo-DBF localizes singlet-oxygen generation to a selected protein, organelle, or subcellular compartment. Visible-light activation oxidizes nearby guanosine residues in RNA, enabling capture by propargylamine and subsequent click-chemistry conjugation to biotin or fluorescent probes for enrichment, imaging, RT-qPCR, or RNA-seq analysis. This reagent supports proximity-based profiling of localized RNAs at nuclear, cytoplasmic, nucleolar, mitochondrial, and other defined cellular sites while preserving native cellular organization. Halo-DBF is well suited for RNA localization studies, transcriptome mapping, organelle-associated RNA analysis, and development of HaloTag-enabled chemical biology workflows.

Product Name	Halo-DBF
Synonyms	Halo-Dibromofluorescein
CAS Number	N/A
Related CAS	N/A
Molecular Formula	C35H34Br2ClNO10
Molecular Weight	823.91
Purity (HPLC)	>= 95%
Solubility	DMSO, DMF
Shipping Conditions	Shipped on gel packs
Storage Conditions	Store at -20°C and protected from light
Shelf Life	12 months after the date of delivery
Regulatory Statement	For Research Use Only

References

(1) Engel, K. L., Lo, H. Y. G., Goering, R., Li, Y., Spitale, R. C., & Matthew Taliaferro, J. (2022). Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq. Nucleic Acids Research, 50(4), E24. https://doi.org/10.1093/nar/gkab1185

(2) Li, Y., Aggarwal, M. B., Nguyen, K., Ke, K., & Spitale, R. C. (2017). Assaying RNA Localization in Situ with Spatially Restricted Nucleobase Oxidation. ACS Chemical Biology, 12(11), 2709–2714. https://doi.org/10.1021/acschembio.7b00519

 

Documents 

Datasheet          MSDS

 

Citations

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