HaloTag is a self-labeling protein derived from the haloalkane dehalogenase DhaA from Rhodococcus rhodochrous. Its active site reacts with chloroalkane substrates to form an irreversible bond. The covalent linkage between the HaloTag ligand and HaloTag protein enables rapid isolation and purification. HaloTag Coumarin Ligand is a cell permeable HaloTag fluorescent ligand and can be used to label intracellular proteins (353Ex/434Em). It is an equivalent of Promega product G8581 and G8582.
| Product Name | HaloTag Coumarin Ligand |
| Synonyms | Coumarin-Halo |
| CAS Number | |
| Related CAS | |
| Molecular Formula | C22H31ClN2O5 |
| Molecular Weight | 438.95 |
| Purity (HPLC) | >= 95% |
| Physical Appearance | Yellow Solid |
| Optical Properties | Abs/Em Maxima: 353/434 nm |
| Solubility | DMSO, DMF |
| Shipping Conditions | Shipped on gel packs |
| Storage Conditions | Store at -20°C and protected from light |
| Shelf Life | 12 months after the date of delivery |
| Regulatory Statement | For Research Use Only |
References
Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00260
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