BGSN3

SNAP-tag is a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. At 20 kDa, SNAP-tag is smaller than HaloTag (33 kDa), potentially reducing interference with the tagged protein’s function. SNAP-tag reacts with covalently with benzylguanine (BG)or chloropyrimidine (CP) derivatives, forming a stable thioether bonds that ensures durability for long-term experiments or harsh conditions. SNAP-tag can be used alongside other tags like CLIP-tag for multicolor labeling. BGSN3 is a SNAP-tag substrate containg an azide group for click chemistry applications. The activities of SNAP-tag and H5 enzymes on BGSN3 are reasonable (IC50=17.8 and 10 ?M, respectively). In click chemistry, azides are commonly used in azide-alkyne cycloaddition reactions, such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC) and the strain-promoted alkyne-azide cycloaddition (SPAAC). These reactions are efficient and selective, forming a triazole ring and often used in drug development, molecular biology, and bioconjugation.

References

Merlo, R., Caprioglio, D., Cillo, M., Valenti, A., Mattossovich, R., Morrone, C., Massarotti, A., Rossi, F., Miggiano, R., Leonardi, A., Minassi, A., & Perugino, G. (2021). The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. Journal of Enzyme Inhibition and Medicinal Chemistry, 36(1), 85–97. https://doi.org/10.1080/14756366.2020.1841182

Documents 

Datasheet          MSDS

Citations

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