BC-CPY

CLIP-tag is a modified version of the SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Unlike the SNAP-tag, which reacts with benzylguanine (BG)or chloropyrimidine (CP) derivatives, the CLIP-tag is engineered to specifically interact with benzylcytosine (BC)derivatives. This modification allows for distinct labeling applications, enabling researchers to use both SNAP-tag and CLIP-tag in the same experiment, providing flexibility in protein labeling and tracking. BC-CPY is a CLIP-tag substrate containing the 6-CPY dye. It forms a covalent bond with the CLIP-tag protein, ensuring stable labeling for long-term tracking. CPY dye (short for carbopyronine, 606Ex/626Em) is a red-emitting fluorophore that emits strong, stable fluorescence in the red spectrum. This makes it ideal for high-resolution imaging techniques like STED (Stimulated Emission Depletion) and SIM (Structured Illumination Microscopy), as well as for tracking proteins in live-cell experiments. The photostability and brightness of CPY make it a valuable tool for detailed imaging of cellular dynamics.

References

Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00266

Documents 

Datasheet          MSDS

Citations

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