BG-Oregon Green

SNAP-tag is a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. At 20 kDa, SNAP-tag is smaller than HaloTag (33 kDa), potentially reducing interference with the tagged protein’s function. SNAP-tag reacts with covalently with benzylguanine (BG)or chloropyrimidine (CP) derivatives, forming a stable thioether bonds that ensures durability for long-term experiments or harsh conditions. SNAP-tag can be used alongside other tags like CLIP-tag for multicolor labeling. BG-Oregon Green is a SNAP-tag substrate containg Oregon Green Dye. It forms a covalent bond with the SNAP-tag protein, ensuring stable labeling for long-term tracking. Oregon Green dye (496Ex/516Em) is structurally similar to fluorescein but modified with additional fluorine atoms to enhance its optical properties. Oregon Green resists fading under prolonged illumination better than fluorescein. It also has higher quantum yield and extinction coefficient, and retains fluorescence in acidic conditions where fluorescein dims.

References

Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00258

Documents 

Datasheet          MSDS

Citations

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