CA-Cy3
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HaloTag is a self-labeling protein tag engineered from the haloalkane dehalogenase DhaA, originally isolated from Rhodococcus rhodochrous. Its active site binds chloroalkane (CA)substrates, forming an irreversible covalent bond. This stable linkage between the HaloTag protein and its ligand enables rapid and efficient isolation and purification of tagged proteins. CA-Cy3 is a HaloTag ligand with Cyanine3 dye. Cy3 (Cyanine 3, 550Ex/570Em) is known for its high fluorescence intensity and photostability, making it useful for both short- and long-term imaging applications. Cy3 can be used in combination with other fluorescent dyes (e.g., Cy5, GFP) to enable multicolor imaging, where multiple targets can be visualized simultaneously.
| Product Name | CA-Cy3 |
| Synonyms | Cy3-Halo, Cy3-CA |
| CAS Number | |
| Related CAS | |
| Molecular Formula | C40H57ClN3O3 |
| Molecular Weight | 663.36 |
| Purity (HPLC) | >= 95% |
| Solubility | DMSO, DMF |
| Shipping Conditions | Shipped on gel packs |
| Storage Conditions | Store at -20°C and protected from light |
| Shelf Life | 12 months after the date of delivery |
| Regulatory Statement | For Research Use Only |
References
Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00267
Documents
Datasheet MSDS
Citations
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