CA-SS-SulfoCy3

HaloTag is a self-labeling protein tag engineered from the haloalkane dehalogenase DhaA, originally isolated from Rhodococcus rhodochrous. Its active site binds chloroalkane (CA)substrates, forming an irreversible covalent bond. This stable linkage between the HaloTag protein and its ligand enables rapid and efficient isolation and purification of tagged proteins. CA-SS-SulfoCy3 is a membrane-impermeable probe that covalently binds to the Halo-tag, emitting in the red spectrum. It features a disulfide bond, allowing the non-internalized probe to be released from the cell surface using the reducing agent sodium 2-mercaptoethanesulfonate (MESNA). Together, CA-SS-SulfoCy3 and CA-SS-SulfoCy5 have been utilized for the simultaneous visualization of multiple internalized cell surface proteins in two colors, exemplified by studies involving G protein-coupled receptors. SulfoCy3(Sulfo-Cyanine3, 548Ex/562Em) is a sulfonated derivative of the Cy3 dye, with the addition of sulfonic acid groups that enhance its solubility in aqueous environments. This dye hass bright fluorescence, high extinction coefficient, and photostability, which allow for sensitive detection in various imaging and analytical techniques. Its fluorescence is relatively pH-insensitive, and it exhibits reduced dye aggregation compared to non-sulfonated cyanine dyes, improving its performance in labeling applications.

References

Roßmann, K., Birke, R., Levitz, J., Jones, B., & Broichhagen, J. (2024). Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization. RSC Chemical Biology. https://doi.org/10.1039/d4cb00209a

Documents 

Datasheet          MSDS

Citations

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