CA-SS-SulfoCy5

HaloTag is a self-labeling protein tag engineered from the haloalkane dehalogenase DhaA, originally isolated from Rhodococcus rhodochrous. Its active site binds chloroalkane (CA)substrates, forming an irreversible covalent bond. This stable linkage between the HaloTag protein and its ligand enables rapid and efficient isolation and purification of tagged proteins. CA-SS-SulfoCy5 is a membrane-impermeable probe that covalently binds to the Halotag, emitting in the far-red spectrum. It features a disulfide bond, allowing the non-internalized probe to be released from the cell surface using the reducing agent sodium 2-mercaptoethanesulfonate (MESNA). Together,CA-SS-SulfoCy5 and CA-SS-SulfoCy3 have been utilized for the simultaneous visualization of multiple internalized cell surface proteins in two colors, exemplified by studies involving G protein-coupled receptors. SulfoCy5(Sulfo-Cyanine5, 650Ex/670Em) is a sulfonated derivative of its parent dye, Cy5, with sulfonic acid groups that enhance its solubility in water and reduce aggregation, making it ideal for aqueous-based applications without requiring organic solvents. Compared to the original Cy5 dye, Sulfo-Cy5 offers similar optical characteristics but with enhanced water solubility and reduced non-specific interactions,

References

Roßmann, K., Birke, R., Levitz, J., Jones, B., & Broichhagen, J. (2024). Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization. RSC Chemical Biology. https://doi.org/10.1039/d4cb00209a

Documents 

Datasheet          MSDS

Citations

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