BC-Map555

CLIP-tag is a modified version of the SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Unlike the SNAP-tag, which reacts with benzylguanine (BG)or chloropyrimidine (CP) derivatives, the CLIP-tag is engineered to specifically interact with benzylcytosine (BC)derivatives. This modification allows for distinct labeling applications, enabling researchers to use both SNAP-tag and CLIP-tag in the same experiment, providing flexibility in protein labeling and tracking. BC-Map555 is a CLIP-tag substrate containg Map555 dye and it forms a covalent bond with the CLIP-tag protein, ensuring stable labeling for long-term tracking. MaP555 (558Ex/578Em) is a small-molecule fluorogenic probe with excellent cell permeability. It is converted from 6-carboxytetramethylrhodamine (6-TAMRA) and has similar spectroscopic properties of 6-TAMRA. MaP555 has an acyl sulfamide structure which favors the spirolactam form, thus has excellent cell permeability. When the probe binds to its cellular targets, MaP555 mainly exists in the fluorescent zwitterion form. The good spectroscopic properties, high cell permeability and outstanding fluorogenicity of MaP555 make it very useful for wash-free, multicolour, live-cell nanoscopy.

References

Wang, L., Tran, M., D’Este, E., Roberti, J., Koch, B., Xue, L., & Johnsson, K. (2020). A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy. Nature Chemistry, 12(2), 165–172. https://doi.org/10.1038/s41557-019-0371-1

Documents 

Datasheet          MSDS

Citations

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