BC-TMR

CLIP-tag is a modified version of the SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Unlike the SNAP-tag, which reacts with benzylguanine (BG)or chloropyrimidine (CP) derivatives, the CLIP-tag is engineered to specifically interact with benzylcytosine (BC)derivatives. This modification allows for distinct labeling applications, enabling researchers to use both SNAP-tag and CLIP-tag in the same experiment, providing flexibility in protein labeling and tracking. BC-TMR is a CLIP-tag substrate containg 6-tetramethylrhodamine dye and it forms a covalent bond with the CLIP-tag protein, ensuring stable labeling for long-term tracking. TMR dye (555Ex/585Em) provides strong red fluorescence, enabling researchers to visualize and track the protein of interest in live cells or tissue using fluorescence microscopy or imaging systems.

References

Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00263

Documents 

Datasheet          MSDS

Citations

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