AzBG

SNAP-tag is a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. At 20 kDa, SNAP-tag is smaller than HaloTag (33 kDa), potentially reducing interference with the tagged protein’s function. SNAP-tag reacts with covalently with benzylguanine (BG)or chloropyrimidine (CP) derivatives, forming a stable thioether bonds that ensures durability for long-term experiments or harsh conditions. SNAP-tag can be used alongside other tags like CLIP-tag for multicolor labeling. BG-Azide is a SNAP-tag substrate containg an azide group for click chemistry applications. It has been studied for labeling of HoxB8-derived macrophages via SNAP-Tag and bioorthogonal chemistry. In click chemistry, azides are commonly used in azide-alkyne cycloaddition reactions, such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC) and the strain-promoted alkyne-azide cycloaddition (SPAAC). These reactions are efficient and selective, forming a triazole ring and often used in drug development, molecular biology, and bioconjugation.

References

Schöning, J., Rösner, L., Depke, D. A., Hüwel, S., Kukhar, A., Schäfers, M., & Rentmeister, A. (2025). Toolbox of Clickable Benzylguanines for Labeling of HoxB8-Derived Macrophages via SNAP-Tag and Bioorthogonal Chemistry. Bioconjugate Chemistry. https://doi.org/10.1021/acs.bioconjchem.4c00364

Documents 

Datasheet          MSDS

Citations

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