BC-FAM

CLIP-tag is a modified version of the SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Unlike the SNAP-tag, which reacts with benzylguanine (BG)or chloropyrimidine (CP) derivatives, the CLIP-tag is engineered to specifically interact with benzylcytosine (BC)derivatives. This modification allows for distinct labeling applications, enabling researchers to use both SNAP-tag and CLIP-tag in the same experiment, providing flexibility in protein labeling and tracking. BC-FAM is a CLIP-tag substrate containg 6-fluorescein dye and it forms a covalent bond with the CLIP-tag protein, ensuring stable labeling for long-term tracking. Fluorescein dye (494Ex/526Em) is one of the most popular green fluorescent reagents used for in situ labeling of peptides, proteins and nucleotides. Fluorescein has relatively high absorptivity, excellent fluorescence quantum yield, good water solubility, and an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

References

Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00264

Documents 

Datasheet          MSDS

Citations

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