BG-SS-SulfoCy5

SNAP-tag is a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. At 20 kDa, SNAP-tag is smaller than HaloTag (33 kDa), potentially reducing interference with the tagged protein’s function. SNAP-tag reacts with covalently with benzylguanine (BG)or chloropyrimidine (CP) derivatives, forming a stable thioether bonds that ensures durability for long-term experiments or harsh conditions. SNAP-tag can be used alongside other tags like CLIP-tag for multicolor labeling. BG-SS-SulfoCy5 is a membrane-impermeable probe that covalently binds to the SNAP-tag, emitting in the far-red spectrum. It features a disulfide bond, allowing the non-internalized probe to be released from the cell surface using the reducing agent sodium 2-mercaptoethanesulfonate (MESNA). Together, BG-SS-SulfoCy5 and BG-SS-SulfoCy3 have been utilized for the simultaneous visualization of multiple internalized cell surface proteins in two colors, exemplified by studies involving G protein-coupled receptors. SulfoCy5(Sulfo-Cyanine5, 650Ex/670Em) is a sulfonated derivative of its parent dye, Cy5, with sulfonic acid groups that enhance its solubility in water and reduce aggregation, making it ideal for aqueous-based applications without requiring organic solvents. Compared to the original Cy5 dye, Sulfo-Cy5 offers similar optical characteristics but with enhanced water solubility and reduced non-specific interactions,

References

Roßmann, K., Birke, R., Levitz, J., Jones, B., & Broichhagen, J. (2024). Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization. RSC Chemical Biology. https://doi.org/10.1039/d4cb00209a

Documents 

Datasheet          MSDS

Citations

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