CP-CPY

SNAP-tag is a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. At 20 kDa, SNAP-tag is smaller than HaloTag (33 kDa), potentially reducing interference with the tagged protein’s function. SNAP-tag reacts with covalently with benzylguanine (BG)or chloropyrimidine (CP) derivatives, forming a stable thioether bonds that ensures durability for long-term experiments or harsh conditions. SNAP-tag can be used alongside other tags like CLIP-tag for multicolor labeling. Chloropyrimidine (CP) derivatives generally exhibit better cell permeability compared to their benzylguanine (BG) derivatives. CP-CPY is a SNAP-tag substrate containg CPY dye. It forms a covalent bond with the SNAP-tag protein, ensuring stable labeling for long-term tracking. CPY dye (short for carbopyronine, 606Ex/626Em) is a red-emitting fluorophore that emits strong, stable fluorescence in the red spectrum. This makes it ideal for high-resolution imaging techniques like STED (Stimulated Emission Depletion) and SIM (Structured Illumination Microscopy), as well as for tracking proteins in live-cell experiments. The photostability and brightness of CPY make it a valuable tool for detailed imaging of cellular dynamics.

References

Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00266

Documents 

Datasheet          MSDS

Citations

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