CP-DOTA-tris(t-Bu)

SNAP-tag is a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. At 20 kDa, SNAP-tag is smaller than HaloTag (33 kDa), potentially reducing interference with the tagged protein’s function. SNAP-tag reacts with covalently with benzylguanine (BG)or chloropyrimidine (CP) derivatives, forming a stable thioether bonds that ensures durability for long-term experiments or harsh conditions. SNAP-tag can be used alongside other tags like CLIP-tag for multicolor labeling. Chloropyrimidine (CP) derivatives generally exhibit better cell permeability compared to their benzylguanine (BG) derivatives. CP-DOTA-tris(t-Bu) is a SNAP-tag substrate containg a precursor of the macrocyclic chelator DOTA. It forms a covalent bond with the SNAP-tag protein, ensuring stable labeling for long-term tracking. DOTA ( 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) consists of a 12-membered tetraaza ring (cyclen) with four nitrogen atoms, each substituted with an acetic acid (-CH?COOH) group. This structure allows DOTA to form highly stable complexes with a variety of metal cations, particularly those with multiple positive charges, by coordinating through its four nitrogens and four carboxylate oxygen atoms. Its exceptional stability and versatility make it a cornerstone in applications like medical imaging, radiotherapy, and biochemical research.

References

Documents 

Datasheet          MSDS

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