5-(and-6)-carboxyl-2,7-dichlorofluorescein diacetate (Carboxy-DCFDA, CDCF) can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2,7-dichlorofluorescein. With a pKof 4.8, this dye can be used as an acidic pH sensor. It has been used as a fluorescent substrate/probe to study the distribution and mechanism of action of multidrug resistance-associated protein 2 (Mrp2).
| Product Name: | 5-(and-6)-carboxyl-2',7'-dichlorofluorescein diacetate |
| Synonyms: | Carboxy-DCFDA |
| CAS Number: | 127770-45-0 |
| Molecular Formula: | C25H14Cl2O9 |
| Molecular Weight: | 529.28 |
| SMILES: | CC(=O)OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C22OC(=O)C3=CC(=CC=C23)C(O)=O)=C1.CC(=O)OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C22OC(=O)C3=CC=C(C=C23)C(O)=O)=C1 |
| Purity (HPLC): | ≥ 95% |
| Shipping Conditions: | room temperature |
| Storage Conditions: | Refrigerated |
| Regulatory Statement: | For Research Use Only |