5-FAM azide



Size: 5mg

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5-FAM azide is an excellent alkyne-reactive fluorescent reagent for labeling azide-containing biological molecules through click chemitry. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.

Product Name: 5-FAM azide
Synonyms: 5-Carboxyfluorescein azide
CAS Number:
Molecular Formula: C24H18N4O6
Molecular Weight: 458.43
Purity (HPLC): ≥ 95%
Shipping Conditions: room temperature
Storage Conditions: Refrigerated
Regulatory Statement: For Research Use Only