The red-fluorescent 5(6)-TAMRA alkyne is an excellent azide-reactive fluorescent reagent for labeling alkyne-containing biological molecules through click chemitry. It has the spectral properties identical to those of TRITC conjugates, thus all the instrument settings of the TRITC conjugates would be applicable to the applications of 5(6)-TAMRA alkyne conjugates. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.
||TAMRA alkyne, 5 and 6-isomer
||5- (and -6)-Carboxytetramethylrhodamine, propargylamide
||For Research Use Only