Hoechst-Cy5 (DNA-binding dye)

Hoechst-Cy5 is a small-molecule DNA-specific fluorophore. It can be used as a fast, simple, and robust DNA stain for high-quality superresolution chromatin imaging on clinically processed tissue samples. In a comparative study of STORM (Stochastic optical reconstruction microscopy) imaging of genomic DNA using tissue section of mouse intestinal tissue prepared with standard clinical protocol, the images from Hoechst-Cy5 STORM showed the best-resolved DNA nanodomains when compared with TOTO-3 Iodide (TOTO-3), NucSpot Live 650 (Live-650), and Hoechst Janelia Fluor 646 (Hoechst-JF646). Hoechst-Cy5 significantly facilitates routine examination of superresolved chromatin structure from various pathological conditions directly on clinically processed tissue. Excitation maximum = 650 nm; emission maximum = 660 nm. Reference: Xu, J., Sun, X., Kim, K., Brand, R. M., Hartman, D., Ma, H., Brand, R. E., Bai, M., & Liu, Y. (2022). Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe. In Sci. Adv (Vol. 8). https://www.science.org DOI: 10.1126/sciadv.abm8293